© 2017 by Star Array Pte Ltd.

TECHNOLOGY

High-throughput Microfluidic PCR Array

Star Array developed a few microfluidic PCR array technologies, which can be used to generate various next generation disease diagnostic solutions and life science research tools.

PCR is a highly sensitive and specific genetic testing technology, and it has been used in many molecular testing workflows. However, increasing the throughput of PCR to a high level is a challenge. Our microfluidic PCR array technology shown in "Loading of PCR Array Chip" demonstrated a highly parallel microfluidic primer loading (not shown) and parallel microfluidic sample loading and reactor sealing on a plastic reactor array.

High-Density Microfluidic PCR Array
 

Figure on the left shows a micro-mold Star Array Pte Ltd  developed to make the high-density microfluidic PCR array.

High-Density Microfluidics PCR Array
 

The Figure on the left shows a high-density Microfluidic PCR array can be expanded to a very large reactor array (86,000 wells) for PCR-based applications, including dPCR and highly parallel PCR with a large number of different PCR primers, each in different wells using Star Array’s microfluidic primer loading technology.

Loading of PCR Array Chip
 

Star Array Highly Parallel PCR Array for High-Throughput Genetic Testing. The PCR array shown on the left is pre-loaded with different primers and probes in different wells (PCR reactors) using a high-throughput microfluidic reagent loading technology.

Detection of Cancer Gene

 

Figures on the left shows the Star Array Pte Ltd Microfluidic PCR array is used for simultaneous detection of 10 human hepatocellular carcinoma (HCC) cancer genes from surgical biopsies

Performance (1)

 

PCR is a highly sensitive and specific genetic testing technology, and it has been used in many molecular testing workflows. However, increasing the throughput of PCR to a high level is a challenge. Our microfluidic PCR array technology shown in the Figure on the left demonstrated a highly parallel microfluidic primer loading (not shown) and parallel microfluidic sample loading and reactor sealing on a plastic reactor array. 

Performance (2)

Figure on the left shows Star Array Highly Parallel PCR Array for High-Throughput Genetic Testing. The PCR array shown in Image 1 is pre-loaded with different primers and probes in different wells (PCR reactors) using a high-throughput microfluidic reagent loading technology.

Single copy PCR test (1024 wells, 125nl well volume) – year 2010. Performed on Star Array Microgene real time PCR machine)
 

The Cq values from 36 positive wells fit a normal distribution with a P value of 0.67. Based on the Poisson distribution the expected estimated range for 39 single molecule PCR is 32.8 to 45.2 copies.